Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast

摘要

We report time courses of the accumulation and loss of 16 fluorescent fusion proteins at sites of clathrin-mediated endocytosis in fission yeast. Mathematical modeling shows that dendritic nucleation hypothesis can account for the kinetics of actin assembly in vivo and disassembly requires actin filament severing along with depolymerization.